Green approach for simultaneous determination of multi-pesticide residue in environmental water samples using excitation-emission matrix fluorescence and multivariate calibration

Pesticides are among the most widespread organic contaminants in aquatic environments. In this work, a new green fluorescence application was proposed for the simultaneous determination of four widely employed pesticides in environmental water samples. To overcome the highly overlapped spectra within the analytes, and with the tissue matrix interferences in complex solutions, we have used the multivariate calibration methods such as parallel factor analysis (PARAFAC) and unfolded partial least squares coupled to residual bilinearization (U-PLS/RBL). These four pesticides can be identified simultaneously, and the correlation coefficients between resolved and actual spectra are all above 0.95. The second-order advantage allowed the determination of four pesticides at the ng mL − 1 level, even in the presence of humic acid (HA). The best results were obtained with the limits of detection of 1.72–18.69 for Carbendazim (CBZ), 0.30–5.19 for carbaryl (CAR), 0.35–6.32 for chlorothalonil (CHL), and 4.92–29.96 for tsumacide (TSU) (ng mL − 1 ), which can fully meet the quantitative detection and analysis requirements of trace pesticides in water samples. The real water sample of Bohai Seawater was used to check the performance of this approach in practical applications, which have achieved good prediction results of U-PLS/RBL. This study demonstrated the proposed method is rapid, accurate, sensitive, low detection limit, and environmentally friendly to determinate multi-pesticide residues in environmental water samples.