Rapid determination of fumonisin (FB1) by syringe SPE coupled with solid-phase fluorescence spectrometry

Fumonisin B 1 is the most prevalent member of a family of toxins, known as fumonisins , which occurs mainly in maize, wheat and other cereals. Due to its hepatotoxic and nephrotoxic in all animal species, very strict regulations have been imposed on the levels of fumonisin B 1 in cereal and cereal-based foods worldwide. In this work, a rapid determination method of fumonisin B 1 by membrane solid phase extraction coupled with solid-phase fluorescence analysis is developed. A rhodamine based fluorescent probe was used for derivatization with fumonisin B 1 . After derivatization and extraction by nylon membrane, the enriched fumonisin B 1 can be detected directly on the membrane without further elution process that is placed in a designed spectra collection device. The established method showed a linear relationship in concentration range of 0.5–5.0 μg/L, with the R 2  = 0.991, and a limit of detection of 0.119 μg/L. Method accuracy was further confirmed using LC-MS method by comparing the detection results of 3 corn powder samples spiked with FB 1 , that demonstrated equivalent results. The results of this study indicated that the proposed method was simple, sensitive, reliable and suitable for trace fumonisins B 1 quantitation in corn-based feeds.