An integrated strategy for production of four anthocyanin compounds from Ribes nigrum L. by deep eutectic solvents and flash chromatography

This study explored an integrated strategy for extraction and preparative separation of four anthocyanins from Ribes nigrum L. using DES-based microwave assisted extraction and flash chromatography. The extraction yield of anthocyanins reached 2.03   mg/g after optimization. An efficient preparative separation process of four main anthocyanins by macroporous resin and flash chromatography was established afterwards. The yields of D3G, D3R, C3G and C3R reached 0.32   mg/g, 0.47   mg/g, 0.18   mg/g and 0.43   mg/g with the purities of more than 95%, respectively. The developed procedure represents an efficient and convenient alternative for the systematic utilization of natural products from plant materials.