New Sesquiterpenoids from the Fermented Broth of Termitomyces albuminosus and Their Anti-Acetylcholinesterase Activity

Termitomyces albuminosusis the symbiotic edible mushroom of termites and cannot be artificially cultivated at present. In the project of exploring its pharmaceutical metabolites by microbial fermentation, four new selinane type sesquiterpenoids—teucdiol C (1), D (2), E (3), and F (4), together with two known sesquiterpenoids teucdiol B (5) and epi-guaidiol A (6)—were obtained from its fermented broth ofT. albuminosus. Their structures were elucidated by the analysis of NMR data, HR Q-TOF MS spectral data, CD, IR, UV, and single crystal X-ray diffraction.Epi-guaidiol A showed obvious anti-acetylcholinesterase activity in a dose-dependent manner. The experimental results displayed thatT. albuminosuspossess the pharmaceutical potential for Alzheimer’s disease, and it was an effective way to dig new pharmaceutical agent ofT. albuminosuswith the microbial fermentation technique.Keywords:Termitomyces albuminosus;selinane;sesquiterpenoids;anti-acetylcholinesterase;microbial fermentation