Highly sensitive determination of L-tyrosine in pig serum based on ultrathin CuS nanosheets composite electrode

Nanometer-sized copper sulfide has remarkable properties such as metal like electrical conductivity and electrocatalytic activity . In this work, ultrathin copper sulfide nanosheets (CuS NS) were synthesized and employed to modify on surface of glassy carbon electrode (GCE) combining with chitosan (CS) and acidified multi-walled carbon nanotubes (F-MWCNTs). Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the shape of CuS NS was hexagon with side length of 13.33 ± 0.67 nm and thickness of 4.50 ± 0.58 nm. The electrochemical characteristics of different nanocomposite modified electrodes were examined by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), indicating that the modified electrode of CuS NS-CS/F-MWCNTs/GCE possessed good electrocatalytic activity towards oxidation of L-tyrosine (L-Tyr). Under the optimal condition, the modified electrode exhibited a wide linear response range for L-Tyr (0.08–1.0 μM) with a detection limit of 4.9 nM. No obvious interferences from coexisted two-fold of L-tryptophan and 50-fold of other amino acids could be observed, indicating its relatively good selectivity. The electrode also had good repeatability, reproducibility and stability. Compared with a commercial instrument analytical method, HPLC , the electrode can be successfully applied to the determination of L-Tyr in pig serums with a recovery rate of 95.7%–102.6%, and its test results are in good agreement with that of HPLC, showing its promising application value.