The effect of amino-functionalized mesoporous bioactive glass on MC3T3-E1 cells in vitro stimulation

The 3-aminopropyltriethoxysilane (APTES) was grafted on mesoporous bioactive glass (MBG) to prepare amino-functionalized mesoporous bioglass (N-MBG) by reflux agitation method. The corresponding cell experiments were performed to evaluate the influences of bioactive glass on MC3T3-E1 cells in vitro stimulation. The results displayed that the initial zeta-potential on the surface of MBG is −0.06 mV, after grafting APTES, changing to a positive charge about +5.30 mV. The surface of N-MBG was distributed positive charge, which promoted cell adhesion and protein adsorption . SEM images and fluorescence micrographs of MC3T3-E1 cells showed that N-MBG had well biocompatibility in vitro compared with that of BG and MBG. In addition to surface properties, N-MBG with lower degradation rate and pH value also had played a crucial role in promoting bone cell proliferation and differentiation. These results suggested that N-MBG would be a promising material for bone tissue regeneration .