ESI-TOF MS analysis of complexes formed between quercetin and five metal ions in hot water and a study into their DNA cleavage activity

Quercetin is a flavonoid compound widely found in vegetables, fruits, and medicinal plants . It has carbonyl and phenolic hydroxyl groups in its structure that can easily form complexes with metal ions . In this study, we reacted quercetin in water at 95 °C with five metal trace elements commonly found in plants, namely calcium, magnesium, zinc, iron(III), and copper. Each supernatant was then examined by electrospray ionization-time-of-flight mass spectrometry (ESI-TOF MS). The results showed that quercetin can form complexes with Ca 2+ , Mg 2+ , Zn 2+ , and Cu 2+ in ratios of 2:1, 3:1, and 3:2. Interestingly, after reaction with Fe 3+ in water at 95 °C, not only can quercetin‑iron(III) complexes be formed in ratios of 2:1 and 3:1, but small amounts of quercetin‑iron(II) complexes can also be formed in the same ratios. Furthermore, DNA cleavage experiments showed that when acting alone, quercetin and Cu 2+ have weak or no cleavage effects on DNA, but the complex formed after reaction in hot water cleaves DNA in a time- and concentration-dependent manner. These results indicate that complexes may form between quercetin and a variety of metal trace elements in a water decoction of plants, and that these metal complexes may be the material basis for the health-promoting and therapeutic effects of edible or medicinal plants.