Multi-templates based molecularly imprinted sodium alginate/MnO2 for simultaneous enantiorecognition of lysine, alanine and cysteine isomers

Sodium alginate (SA) was electrodeposited onto the surface of a glassy carbon electrode (GCE), and then l -lysine ( l -Lys), l -alanine ( l -Ala) and l -cysteine ( l -Cys) were simultaneously introduced to the surface of SA via electrostatic attractions. γ-MnO 2 film was deposited potentiostatically onto the multi-templates immobilized SA from an aqueous mixture of K 2 SO 4 and MnSO 4 . The templates of l -Lys, l -Ala and l -Cys were then oxidized and removed by cyclic voltammetry (CV) while maintaining the crystalline structures of SA and MnO 2 . Finally, the molecularly imprinted SA/MnO 2 was successfully applied in the simultaneous recognition of the isomers of Lys, Ala and Cys by differential pulse voltammetry (DPV). This work opens a new avenue in the construction of SA-based multi-templates molecularly imprinted systems for electrochemical simultaneous recognition of the isomers of several chiral amino acids .