One-Pot Electrodeposition of NiS Nanoparticles as an Efficient Electrode for Nonenzymatic H2O2 and Glucose Sensors

In this paper, a new and one-pot electrodeposition method was expanded for the preparation of NiS nanoparticles-based electrochemical biosensor using metal-ion complexes as a precursor. Thioacetamide was used to control the production rate of NiS nanoparticles for the first time. The proposed electrochemical sensor was characterized by energy dispersive X-ray spectroscopy (EDX), field emission scanning electron microscope (FESEM), cyclic voltammograms (CV), and electrochemical impedance spectra (EIS). Experiment parameters were optimized. Under the optimized condition, the prepared NiS-based biosensor exhibited excellent electrocatalytic oxidation of H 2 O 2 and glucose due to their small size. It provided fast and sensitive strategy for detecting H 2 O 2 and glucose in the range of 1–5000 and 1–1000 $$ μ $\mu$ M. The detection limit of 0.257 and 0.3 $$ μ $\mu$ M was obtained for H 2 O 2 and glucose. The mechanisms were also analyzed. The proposed biosensor exhibited excellent anti-interference and repeatability. Furthermore, it was applied in the actual sample analysis, such as human blood serum.