Simultaneous Detection of Listeria monocytogenes and Salmonella typhimurium by a SERS-Based Lateral Flow Immunochromatographic Assay

Foodborne diseases arise from Listeria monocytogenes ( L. monocytogenes ) and Salmonella typhimurium ( S. typhimurium ) are global public health problems, influencing both developing and developed countries. Therefore, their rapid and accurate detection is of much concern in food safety and point-of-care diagnostics. In this study, a surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) for simultaneous detection of them was developed. Under optimal conditions, the proposed assay showed good linear responses in the ranges of 10 2 –10 7 cfu/mL and 10 2 –10 7 cfu/mL with limits of detection of 75 cfu/mL and 75 cfu/mL, respectively, for L. monocytogenes and S. typhimurium , respectively. The practicality of the LFA in real food sample (milk) was also validated. Overall, the proposed assay is promising and has a good chance to be employed for multiple pathogen detection in food products.