One-step Electrodeposition of Tris(hydroxymethyl) Aminomethane – Prussian Blue on Screen-printed Electrode for Highly Efficient Detection of Glycosylated Hemoglobin

In this work, the modified Prussian blue (PB) film showed more stable performance in alkaline solution by one-step electrodepositon of PB with tris(hydroxymethyl) aminomethane (Tris) on screen-printed electrode (SPE). The morphology and structure of the modified Tris-PB/SPE was characterized by scanning electronic microscopy, infra spectroscopy, Raman spectroscopy and X-ray diffraction. It was inferred that the Tris particles embedded in the PB deposit layer resulted in the change of PB structure and improve its stability in alkaline solution. And then, the modified Tris-PB/SPE was applied in the detection of Glycosylated hemoglobin (HbA1c). The optimum experimental conditions are pH 7.5, 100 mV/s, 4 μL FAOD and 5 min reaction time. The linearship of HbA1c is i=22.90 C+101.9 in the range of 0.1–2 mmol/L. Comparing with PB/SPE, Tris-PB/SPE shows better sensitivity and recovery.