A colorimetric mercury(II) assay based on the Hg(II)-stimulated peroxidase mimicking activity of a nanocomposite prepared from graphitic carbon nitride and gold nanoparticles

A one-step reduction method was used for the preparation of stable graphitic carbon nitride-gold nanoparticles (g-C 3 N 4 -Au) nanocomposites from ultrathin g-C 3 N 4 nanosheets and chloroauric acid by using NaBH 4 as a reducing agent under ultrasonication. The nanocomposites were characterized by transmission electron microscopy, X-ray photoelectron spectroscopy, UV-Vis absorption and fluorescence spectroscopy etc. The results revealed that the gold nanoparticles (AuNPs) are uniformly formed on the g-C 3 N 4 nanosheets. It is found that the peroxidase-like catalytic activity of this nanocomposite for the oxidation of 3,3′,5,5′-tetramethylbenzidine by H 2 O 2 to form a blue-colored product is strongly enhanced in the presence of Hg(II). Based on this phenomenon, a sensitive “turn-on” colorimetric assay for Hg(II) was developed that works at physiological pH values. Under optimal conditions, the absorption signal at 652 nm increases linearly with Hg(II) concentration in the range from 5 to 500 nM. A detection limit as low as 3.0 nM was achieved. This assay has excellent selectivity over other metal ions. It was successfully applied to the determination of Hg(II) in real water samples. The method is cost-effective, rapid, and allows for visual detection. The nanocomposite composed of graphitic carbon nitride (g-C 3 N 4 ) and gold nanoparticles (g-C 3 N 4 -AuNPs) can catalyze tetramethylbenzidine (TMB) oxidation by H 2 O 2 to produce light-blue product (oxTMB). The peroxidase-like activity of g-C 3 N 4 -AuNPs can be greatly enhanced by Hg 2+ , thus increases the amount of the blue product formed.