Radiolabeling of DNA nanostructure with 99mTc using DTPA as a chelate

In the field of nanotechnology, DNA-based nanoscale materials have facilitated the construction of DNA polyhedrons with different shapes and sizes by using predictable base pairing and highly tunable conformation. In this study DNA bipyramid nanostructures with one arm chain (T20-DBNs) were successfully prepared in a single annealing procedure. 99m Tc-A20 ssDNA was obtained by radiolabeled DTPA-A20 with 99m Tc, and then 99m Tc-DTPA-DBNs were obtained by hybridizing T20-DBNs with 99m Tc-A20 ssDNA. We focused on studying a method of 99m Tc radiolabeling DNA nanostructures with DTPA as a chelate, and hoped to develop a new SPECT molecular imaging probe based on DNA nanostructures.