In vivo analysis of two new fungicides in mung bean sprouts by solid phase microextraction-gas chromatography-mass spectrometry

A novel solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC–MS) method was developed to quantify two new fungicide residuals (Y13149, Y12196) in mung bean sprouts. With a stable and biocompatible elcetrospinning nanofiber (polystyrene/graphene@silica, PS/G@SiO 2 ) as coating, the SPME fiber was directly inserted into the stem of mung bean sprout to in-situ in-vivo sampling and extraction, followed by GC–MS analysis. Under the optimal conditions, satisfactory average recoveries of 99% and 72% were obtained for Y13149 and Y12196 with the relative standard deviations (RSDs) under 16.3%, indicating good precision and anti-matrix ability of the method. The result also exhibited low detection limit (0.06–0.08 μg·L −1 ) and wide liner range (0.3–100 μg·L −1 ) with the correlation coefficients (R 2 ) of 0.9989. The established method was applied successfully to trace the accumulation and distribution of fungicides in mung bean sprouts, and it provides a simple, rapid and reliable quantitative method for food analysis .