Dual layer collagen-GAG conduit that mimic vascular scaffold and promote blood vessel cells adhesion, proliferation and elongation

A dual layer conduit, which consists of collagen I-hyaluronic acid (Col-HA, external layer) and collagen I-heparin (Col-HP, inner layer), has been developed. This conduit not only meets the structural requirement of nature blood vessel, but also presents favourable mechanical performance after crosslinking via EDC. In addition, its biological properties are evaluated through culturing with fibroblast cell (Cos-7) on Col-HA surface and vascular endothelial cell (HMEC) on Col-HP layer. Results demonstrate that the conduit can support Cos-7 and HMEC adhesion, proliferation and elongation on respective side. This dual layer conduit would be a new choice of artificial vascular scaffold and improve blood vessel regeneration for tissue engineering.