Self-assembled sponge-like hydroxyapatite induced by modified articular cartilage membrane template

Using modified pig bone articular cartilage membrane as template, a sponge-like hydroxyapatite (HA) scaffold material was in-situ synthesized by self-assembly in simulated body fluid. Its crystallographic structure and composition were studied by SEM, XRD , FTIR and TEM, which showed that the synthesized sample was carbonated HA crystals and the three-dimensional interconnected sponge-like structure consists of massive nano hydroxyapatite plates. The physicochemical properties of the samples were tested and the results indicated that the sponge-like HA had high density, porosity and specific surface. Experimental cell culture results also showed that sponge-like HA greatly increases the bioactivity, osteoconductivity and differentiation of human osteosarcoma MG-63 cells.