Extraction and Analysis of Gigantol from Dendrobium officinale with Response Surface Methodology

In order to optimize the extraction of gigantol fromDendrobium officinale, the influence of methanol concentration, ultrasonic temperature, and liquid ratio on extraction efficiency was analysed by the response surface analysis method. The results show that the extraction rate reached a maximum when the methanol concentration was 92.98%, the solid-liquid ratio was 27.2 mL/g, and the extraction temperature was 41.41 °C. The content of gigantol ofDendrobium officinalein leaves was significantly higher than that in stems, reaching 4.7942 μg/g. The content of gigantol inDendrobium huoshanensisFengdou was significantly higher than that of other species of Fengdou. This experiment has practical significance for improving the utilization rate ofDendrobium officinale, and provides a reference for the study of the pharmacological and biological activity of gigantol.Keywords:Dendrobium officinale;gigantol;response surface methodology;ultrasonic-assisted extraction