Poly(3,6-diamino-9-ethylcarbazole) based molecularly imprinted polymer sensor for ultra-sensitive and selective detection of 17-β-estradiol in biological fluids

In this work, we reported the synthesis of 3, 6-diamino-9-ethylcarbazole and its application as a new monomer for preparation of molecularly imprinted polymer (MIP) electrochemical sensor . The as prepared MIP sensor exhibited ultrahigh sensitivity and selectivity for the detection of 17-β-estradiol in attomolar levels (1 × 10 –18   mol   L −1 ). The sensor works by detecting the change of the interfacial impedance that is derived from recognition of 17-β-estradiol on the MIP layer. The MIP sensor based on 3, 6-diamino-9-ethylcarbazole monomer revealed better performance than that of unmodified carbazole monomer. The monomer/template ratio, electropolymerization scanning cycles, and the incubation pH values were optimised in order to obtain the best detection efficiency. Under the optimised condition, the MIP sensor exhibits a wide linear range from 1   aM to 10   μM (1 × 10 –18 ̶ 1 × 10 −5   mol   L −1 ). A low detection limit of 0.36   aM (3.6 × 10 –19   mol   L −1 ) and a good selectivity towards structurally similar compounds were obtained. The proposed MIP sensor also exhibits long-term stability and applicability in human serum samples. These advantages enabled this MIP sensor to be a promising alternative of electrochemical sensor and may be extended to detection of other endogenous compounds .