Mn2+-doped NaYF4:Yb,Er upconversion nanoparticles for detection of uric acid based on the Fenton reaction

A novel fluorescence method for the determination of hydrogen peroxide (H 2 O 2 ) and uric acid (UA) was developed. The procedure was based on the hydroxyl radicals (·OH), which effectively quenched the fluorescence of the Mn 2+ -doped NaYF 4 :Yb,Er upconversion nanoparticles (UCNPs). Based on the property of Mn 2+ -doped NaYF 4 :Yb,Er upconversion nanoparticles, the Fenton reaction and enzymatic reaction of uric acid, this method could be used for highly sensitive detection of H 2 O 2 and uric acid. Under optimal conditions, we observed that the fluorescence quenching signal showed good linearity with the H 2 O 2 concentration in the range of 3.00 × 10 -8 M ~ 6.00×10 -5 M, and the detection limit of this assay was 1.30×10 -8 M. Meanwhile, the linear concentration range for UA was 4.00 × 10 -9 M ~ 1.00×10 -5 M, and the lower limit of detection was 1.90×10 -9 M. Furthermore, the developed method was successfully used for the determination of UA levels in human serum with satisfactory results.