The Multi-Template Molecularly Imprinted Polymer Based on SBA-15 for Selective Separation and Determination of Panax notoginseng Saponins Simultaneously in Biological Samples

The feasible, reliable and selective multi-template molecularly imprinted polymers (MT-MIPs) based on SBA-15 (SBA-15@MT-MIPs) for the selective separation and determination of the trace level of ginsenoside Rb1(Rb1), ginsenoside Rg1(Rg1) and notoginsenoside R1(R1) simultaneously from biological samples were developed. The polymers were constructed by SBA-15 as support, Rb1, Rg1, R1as multi-template, acrylamide (AM) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The new synthetic SBA-15@MT-MIPs were satisfactorily applied to solid-phase extraction (SPE) coupled with high performance liquid chromatography (HPLC) for the separation and determination of trace Rb1, Rg1and R1in plasma samples. Under the optimized conditions, the limits of detection (LODs) and quantitation (LOQs) of the proposed method for Rb1, Rg1and R1were in the range of 0.63–0.75 ng·mL−1and 2.1–2.5 ng·mL−1, respectively. The recoveries of R1, Rb1and Rg1were obtained between 93.4% and 104.3% with relative standard deviations (RSDs) in the range of 3.3–4.2%. All results show that the obtained SBA-15@MT-MIPs could be a promising prospect for the practical application in the selective separation and enrichment of tracePanax notoginsengsaponins (PNS) in the biological samples.Keywords:multi-template molecularly imprinted polymers;SBA-15;Panax notoginsengsaponins;separation and determination;solid-phase extraction