A novel high-yield process of phospholipase D-mediated phosphatidylserine production with cyclopentyl methyl ether

In this study, an eco-friendly and high-efficient method for the bioconversion of phosphatidylserine (PS) from phosphatidylcholine (PC) and l -serine was developed. After the strain screening, the Streptomyces chromofuscus phospholipase D ( Sc PLD) was chosen and expressed in Escherichia coli BL21 with pET28a (+). After optimization, the maximal specific productivity and Sc PLD hydrolysis activity reached 9049.6   U/g and 10.6   U/mL, respectively, while induction with 2   g/L lactose, at 25   °C for 12   h. The maximal conversions of PC to PS was 93.4% with a final PS concentration of 106.2   g/L (53.1   g/L/h) under theoptimal conditions: 0.153   M PC in CPME, 2.3   M l-ser in sodium acetate buffer, 2.0   g lyophilized cells/mol PC, 100   mM Ca 2+ , 40   °C, and 2   h of incubation. After scale-up to 1   L, the conversions and yield maintained at 85.4% and 97.2   g/L. Therefore, the enzymatic process showed great potential for industrialization of PS.