A simple and selective resonance Rayleigh scattering-energy transfer spectral method for determination of trace neomycin sulfate using Cu2O particle as probe

The stable Cu 2 O nanocubic (Cu 2 ONC) sol was prepared, based on graphene oxide (GO) catalysis of glucose-Fehling's reagent reaction, and its absorption and resonance Rayleigh scattering (RRS) spectra, transmission electron microscopy (TEM) and energy dispersive spectroscopy (EDS) were examined. Using the as-prepared Cu 2 ONC as RRS probe, and coupling with the neomycin sulfate (NEO) complex reaction , a new, simple, sensitive and selective RRS-energy transfer (RRS-ET) method was established for detection of neomycin sulfate, with a linear range of 1.4–112   μM and a detection limit of 0.4   μM. The method has been applied to the detection of neomycin sulfate in samples with satisfactory results.