Preparation of hemin polymer monolithic column and its application in the separation of proteins and small molecules

A poly(hemin-co-C16-co-TEGDA) monolith was prepared using hemin and 1-hexadecene (C16) as monomers; triethylene glycol dimethacrylate (TEGDA) as crosslinking agent; N,N-dimethylformamide and isopropyl alcohol as porogens; and dibenzoyl peroxide and N,N-dimethyl aniline as initiators in 50 × 4.6 mm i.d. stainless steel column. The morphology and structure of the monolith were characterized by scanning electron microscopy and nitrogen adsorption/desorption isotherm measurement, respectively. Compared with poly(C16-co-TEGDA) monolith, poly(hemin-co-C16-co-TEGDA) monolith had better mechanical properties, higher permeability, and better separation ability. The prepared monolith was used to separate not only proteins but also small molecules.