Selective recognization of dicyandiamide in bovine milk by mesoporous silica SBA-15 supported dicyandiamide imprinted polymer based on surface molecularly imprinting technique

In this work, a highly selective molecular imprinted polymer (SBA-15@MIP) was prepared using dicyandiamide (DCD) as the template molecule and mesoporous silica SBA-15 as the carrier. The resulting polymers were characterized by scanning electron microscopy and FT-IR spectra. A highly ordered mesoporous nanostructure was observed for the fabricated polymer. The performance tests showed that the adsorption capacity of SBA-15@MIP for DCD was higher than those of non-imprinted mesoporous silica polymer (SBA-15@NIP) and SBA-15. SBA-15@MIP was successfully used as the solid-phase extraction (SPE) sorbent coupled with HPLC for trace DCD determination in bovine milk and milk powder. Under selected conditions, a favorable linearity between 1   μg/mL and 100   μg/mL was obtained and the detection limit was estimated to be 0.08   μg/mL (bovine milk) and 0.10   μg/mL (milk powder). These results indicated that SBA-15@MIP could be used as an efficient SPE adsorbent for enrichment of DCD in bovine milk and milk powder.