Preparation of the AOPAN-poly(HEMA) nanofibers via the atom transfer radical polymerization method and their application for laccase immobilization

AOPAN nanofibers were prepared by electrospinning and amidoxime modification, subsequently, HEMA was used as the monomers for surface grafting via atom transfer radical polymerization, followed by coordination with Cu(II) ions, thereafter, the nanofibers AOPAN-poly(HEMA)-Cu(II) were explored as the novel support for laccase immobilization. Scanning electron microscopy was used to visualize the morphology of the nanofibers, and Fourier transform infrared spectroscopy was used to provide information on the surface chemistry of nanofibers. At the same time, the optimization of immobilization conditions and the relative properties of the immobilized laccase were also studied in this paper. The study showed the largest amount of immobilized laccase while the reaction time of atom transfer radical polymerization was 4 h. The immobilized laccase showed a better stability resistance to temperature and pH change, and the initial activity of immobilized laccase retained (60.3 ± 3.1)%, that of the free laccase retained only (21.3 ± 2.1)% when stored at 4℃ for 24 days. Immobilized laccase maintained its initial activity after 10 repeated batches of 64.5%.