Catalytic Performance of Oligonucleotide-Templated Pt Nanozyme Evaluated by Laccase Substrates

Abstract Laccases are effective native biocatalysts for the oxidation of o -/ p -diphenols, aminophenols, polyphenols and polyamines. To overcome the intrinsic limitations of native laccase involving pH, temperature and storage, highly dispersive Pt nanoparticles were synthesized by employing different oligonucleotides as stabilizing agents including A 10 , T 10 , C 10 and G 10 , and their catalytic activities were assessed in the oxidation of laccase substrates under ambient air. This kind of Pt nanozyme displays high stability in the range of 20–90 °C and pH 3–9, beyond the range for native laccase. The laccase-like activities of Pt nanoparticles are greatly associated with the size distribution and the surface charge, which can be easily regulated by the sequence composition and the molar ratio of [precursor]/[template]. The most efficient C 10 -templated Pt nanozyme with 4.6 nm in size, exhibit three times higher affinity toward 2,4-dichlorophenol than that of native laccase. DNA-stabilized Pt nanoparticles display excellent catalytic performance in the oxidation of a wide range of substrates including dopamine, catechol, hydroquinone and p -phenylenediamine. This study paves a way to explore artificial nanoparticles to replace native laccase in the fields of biosensing and green synthesis. Graphical Abstract