β-AgVO3 Nanorods as Peroxidase Mimetic for Colorimetric Determination of Glucose

β-AgVO 3 nanorods have been demonstrated to exhibit intrinsic peroxidase-like activity. The oxidation of glucose can be catalyzed by glucose oxidase (GO x ) to generate H 2 O 2 in the presence of O 2 . The β-AgVO 3 nanorods can catalytically oxidize peroxidase substrates including o -phenylenediamine (OPD), 3,3′,5,5′-tetramethylbenzidine (TMB), and diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) by H 2 O 2 to produce typical color reactions: OPD from colorless to orange, TMB from colorless to blue, and ABTS from colorless to green. The catalyzed reaction by the β-AgVO 3 nanorods was found to follow the characteristic Michaelis–Menten kinetics. Compared with horseradish peroxidase and AgVO 3 nanobelts, β-AgVO 3 nanorods showed a higher affinity for TMB with a lower Michaelis–Menten constant ( K m ) value (0.04118 mM) at the optimal condition. Taking advantage of their high catalytic activity, the as-synthesized β-AgVO 3 nanorods were utilized to develop a colorimetric sensor for the determination of glucose. The linear range for glucose was 1.25–60 μM with the lower detection limit of 0.5 μM. The simple and sensitive GO x -β–AgVO 3 nanorods–TMB sensing system shows great promise for applications in the pharmaceutical, clinical, and biosensor detection of glucose.