Cytotoxicity of gold nanoparticles with different structures and surface-anchored chiral polymers

Nanoparticles (NPs) can have profound effects on cell biology. However, the potential adverse effects of gold nanoparticles (AuNPs) with different surface chirality and structures have not been elucidated. In this study, monolayers of poly(acryloyl- l ( d )-valine ( l ( d )-PAV) chiral molecules were anchored on the surfaces of gold nanocubes (AuNCs) and nanooctahedras (AuNOs), respectively. The l -PAV-AuNCs and d -PAV-AuNCs, or the l -PAV-AuNOs and d -PAV-AuNOs, had identical physicochemical properties in terms of size, morphology and ligand density except of the reverse molecular chirality on the particle surfaces, respectively. The l -PAV capped AuNCs and AuNOs exhibited larger cytotoxicity to A549 cells than the D-PAV coated ones, and the PAV-AuNOs had larger cytotoxicity than PAV-AuNCs when being capped with the same type of enantiomers , respectively. The cytotoxicity was positively correlated with the cellular uptake amount, and thereby the production of intracellular reactive oxygen species (ROS). Statement of Significance • Gold nanoparticles with different structure and surface chirality are fabricated. • The structure and surface chirality at the nanoscale can influence cytotoxicity and genotoxicity. • A new perspective on designing nanoparticles for drug delivery, bioimaging and diagnosis.