Development and cytotoxicity of Schiff base derivative as a fluorescence probe for the detection of l-Arginine

Inspired from biological counter parts, chemical modification of Schiff base derivatives with function groups may provide a highly efficient method to detect amino acids . Therefore, a fluorescent probe involving Schiff base and hydroxyl group has been designed and prepared, which showed high response and specificity for Arginine (Arg) among normal eighteen standard kinds of amino acids (Alanine, Valine , Leucine , Isoleucine , Methionine , Asparticacid, Glutamicacid, Arginine, Glycine, Serine , Threonine , Asparagine , Phenylalanine , Histidine , Tryptophan , Proline , Lysine, Glutamine, Tyrosine and Cysteine). Furthermore, theoretical investigation further illustrated the possible binding mode in the host-guest interaction and the roles of molecular frontier orbitals in molecular interplay. In addition, the synthesized fluorescent probe exhibited high binding ability for Arg and low cytotoxicity to MCF-7 cells over a concentration range of 0–200 μg mL −1 which can be also used as a biosensor for the Arg detection in vivo.