Electrochemical Sensor Based on a Novel Pt−Au Bimetallic Nanoclusters Decorated on Reduced Graphene Oxide for Sensitive Detection of Ofloxacin

Pt−Au nanoclusters decorated on the surface of reduced graphene oxide (rGO/Pt−Au) was facilely prepared by one-pot electrochemical reduction. The morphology and composition of rGO/Pt−Au composites had been characterized by scanning electron microscopy (SEM) coupled with energy-dispersive X-ray spectrometry (EDX), fourier transform-infrared spectroscopy (FT-IR) and electrochemical methods. Ofloxacin is a member of synthetic quinolones which has been widely used for the treatment of common diseases in humans and animals. The performance of the rGO/Pt−Au nanocomposite toward the oxidation of ofloxacin was compared with the other similar nanostructures like rGO/Pt and rGO/Au. In the optimized conditions, two linear calibration curves were obtained, from 0.08 to 10 μM and 10 to 100 μM ofloxacin. A detection limit of 0.05 μM ofloxacin was observed at pH 5.7 for the GCE/rGO/Pt−Au. The proposed sensor was successfully applied to determine ofloxacin in tablets and human urine samples and the results were satisfactory.