Biosensor based on bacterial cellulose-Au nanoparticles electrode modified with laccase for hydroquinone detection

Bacterial cellulose-gold nanoparticles (BC-AuNPs) hybrid nanofibers were synthesized by in-situ chemical reduction. The obtained BC-AuNPs nanofibers were characterized by a series of analytical techniques. The results revealed that gold nanoparticles (AuNPs) were successfully deposited on the surfaces of bacterial cellulose nanofibers. Then, the as-prepared BC-AuNPs hybrid nanofibers were attached onto the electrode surface followed by adding a certain amount of laccase (Lac) and Nafion to construct novel a biosensing platform. The prepared electrochemical biosensor was employed to detect hydroquinone. The sensor showed excellent electrocatalysis towards dopamine with low detection limit (5.71   nM), and wide linear range (30–100   nM). Moreover, the biosensor also showed good repeatability, reproducibility, selectivity and stability and was successfully used in the detection of hydroquinone in lake water samples, thus providing a promising method for hydroquinone detection in water environments.