An efficient Horseradish peroxidase chemiluminescence biosensor with surface imprinting based on phenylboronic acid functionalized ionic liquid@magnetic graphene oxide

Mainly, ionic liquid (IL) was used as functional bridging agent and surface modifier to obtain phenylboronic acid modified-IL functionalized GO@Fe 3 O 4 nanoparticles (GO@Fe 3 O 4 @IL/PBA) with reversible covalent binding properties with cis -diol containing compounds particularly. Then GO@Fe 3 O 4 @IL/PBA with the properties of high surface area and easy separation was designed as supporting material to prepare Horseradish Peroxidase (HRP) SMIP which then was characterized by SEM , XRD and FTIR . The adsorption capacity of GO@Fe 3 O 4 @IL/PBA-SMIP to HRP was researched to be 8.8   g/g, and the adsorption model and adsorption kinetics of GO@Fe 3 O 4 @IL/PBA-SMIP to HRP followed Langmuir adsorption isotherm and pseudo second-order model while the washing process was superior with acetic acid than NaOH solutions. Possessing the merit of improving selectivity, GO@Fe 3 O 4 @IL/PBA-SMIP was introduced into CL biosensor which could linearly respond to HRP in the range of 1.0   ×   10 −4 –8.0   ×   10 −3   mg/mL with a detection limit 2.9   ×   10 −5   mg/mL. Finally, the proposed GO@Fe 3 O 4 @IL/PBA-SMIP-CL biosensor was used to detect the HRP in samples with satisfied results and the recoveries were ranged from 87% to 107%.