Electrochemical non-enzyme sensor for detecting clenbuterol (CLB) based on MoS2-Au-PEI-hemin layered nanocomposites

Clenbuterol (CLB), a kind of β 2 -adrenergic agonists, could disrupt cardiovascular and central nervous system. In this work, a new electrochemical non-enzyme sensor for detecting Clenbuterol (CLB) was fabricated based on MoS 2 -Au-PEI-hemin layered nanocomposites . The two-dimensional (2-D) MoS 2 nanosheets were first in-situ assembled with Au nanoparticles , and polyethylenimine (PEI), then hemin molecules were immobilized onto the MoS 2 -Au-PEI film-modified glassy carbon electrode (GCE) via amide bond. Scanning electron microscopy (SEM) and Zeta potential measurements were employed to characterize the MoS 2 -based nanomaterials . Cyclic voltammetry (CV) was used to investigate electrochemical activity of the immobilized hemin on the modified electrode . Upon the optimum conditions, the proposed electrochemical sensor showed an excellent response for CLB including a wide linear ranging from 10   ng/mL to 2   μg/mL and a detection limit (LOD) of 1.92   ng/mL CLB (S/N=3) with favorable reproducibility and stability. Furthermore, this presented method could be feasible for determining CLB in the real pork samples.