Immobilization of pectinase onto Fe3O4@SiO2–NH2 and its activity and stability

Fe 3 O 4 magnetic nanometer particles (MNPS) with a diameter of 30–40   nm and coated by ammoniated silicon dioxides were developed by sol–gel method. The X-ray diffraction (XRD) pattern indicates that the Fe 3 O 4 is wrapped in the amorphous SiO 2 , and the Fourier-transform infrared spectroscopy (FT-IR) analysis confirmed the NH 2 group had been successfully introduced onto the surface of Fe 3 O 4 @SiO 2 nanoparticles. The main factors related to the immobilization process of pectinase onto the magnetic support were optimized by multi-factor orthogonal experiments, and the recommended combination is that 3% glutaraldehyde (coupling agent), pH   =   8.0, 50   °C, and cross-linking time 8   h. Scanning electron microscope (SEM) and FT-IR analyses confirmed that the pectinase had been immobilized onto the surface of the amino modified nanoparticles. The storage stability and reusability of the pectinase were enhanced by the immobilization that 64.4% of the total starting activity was retrieved after 7 recycling batches and only about 21.1% of the total activity was lost after 30-day storage. For the application of the immobilized pectinase, the optimal pH and temperature were 8.5 and 55   °C, respectively.