Detection of 6-Thioguanine by surface-enhanced Raman scattering spectroscopy using silver nanoparticles-coated silicon wafer

In this paper, silver nanoparticles-coated silicon wafer (AgNPs@Si) was used as surface-enhanced Raman scattering (SERS) substrate for detection of 6-Thioguanine (6-TG). The influence of experimental parameters including pH value and soaking time on SERS performance were examined. The SERS characteristic peak of 6-TG at 1303   cm −1 was selected for quantity analysis. With the 521   cm −1 band of silicon as internal standard, band intensity ratio of 6-TG to silicon, that was I 1303 / I 521 , could be used for quantification. Under the optimum conditions, I 1303 / I 521 exhibited a linear relationship with the concentration of 6-TG in the range of 0.1–15.0   μmol   L −1 and detection limit (S/N   =   3) was 0.065   μmol   L −1 . The corresponding correlation coefficient of the linear equation was 0.998. This method based on AgNPs@Si was successfully applied to the detection of 6-TG in two kinds of commercial tablets.