Biocatalytic Resolution of Rac-α-Ethyl-2-Oxo-Pyrrolidineacetic Acid Methyl Ester by Immobilized Recombinant Bacillus cereus Esterase

A new esterase-producing strain ( Bacillus cereus WZZ001) which exhibiting high hydrolytic activity and excellent enantioselectivity on rac -α-ethyl-2-oxo-pyrrolidineacetic acid methyl ester ( R , S - 1 ) has been isolated from soil sample by our laboratory. In this study, the stereoselective hydrolysis of ( R , S - 1 ) was performed using the recombinant Bacillus cereus esterase which expressed in Escherichia coli BL21 (DE3). Under the optimized conditions of pH 8.0, 35 °C, and concentration of substrate 400 mM, a successful enzymatic resolution was achieved with an e.e. s of 99.5 % and conversion of 49 %. Immobilization considerably increased the reusability of the recombinant esterase; the immobilized enzyme showed excellent reusability during 6 cycles of repeated 2 h reactions at 35 °C. Thereby, it makes the recombinant B. cereus esterase a usable biocatalyst for industrial application.