Development of an Immunochromatographic Strip for Rapid Detection of Pantoea stewartii subsp. stewartii

A rapid, simple, sensitive, and specific immunochromatographic test strip was developed for the detection ofPantoea stewartii subsp. stewartii(Pss) in corn seed which was soaked overnight and then centrifuged for precipitate re-dissolved as samples. A pair of sensitive monoclonal antibodies for the immunochromatographic test strip was generated by mice immunization and cell fusion. Under optimized conditions, the lower detection limit of the strips for Pss was 1 × 105cfu/mL both in 0.01 M phosphate buffer solution and corn seed samples, with no cross-reactivity with other common plant pathogens. The developed strip is useful and rapid for the detection of Pss in corn seed samples.Keywords:Pantoea stewartii subsp. stewartii(Pss);immunochromatographic test strip;monoclonal antibody