On-column enrichment and surface-enhanced Raman scattering detection in nanoparticles functionalized porous capillary monolith

A monolithic column functionalized with gold nanoparticles (GNPs) was designed to provide ultrasensitive detection with surface-enhanced Raman scattering (SERS). The monolithic column based on poly (glycidyl methacrylate- co -ethylene dimethacrylate) (GMA- co -EDMA) was served as the enrichment sorbent to concentrate GNPs and targets. We found that 60   nm GNPs-functionalized monolithic columns demonstrated the best SERS enhancement, and the lowest detectable concentration for PATP and CV could be achieved at 10 −7 and 10 −11   M, respectively. Moreover, the columns exhibit a good reproducibility in both spot-to-spot (∼10%) and batch-to-batch (∼15%). The SERS monolithic column with a high sensitivity and reproducibility has a great potential in the field-based rapid detection of targets in complex real-world samples.