New method for fabricating an α-fetoprotein affinity monolithic polymer array

Bisphenol A based epoxy acrylate (BABEA), a commercial UV-curable material, was introduced as a crosslinker for the fabrication of an epoxy-functionalized monolithic polymer array through UV-initiated copolymerization with glycidyl methacrylate as the functional monomer and poly(ethylene glycol) 200 as the porogen. Scanning electron microscopy images showed that the monolithic poly(bisphenol A based epoxy acrylate- co -glycidyl methacrylate) [poly(BABEA- co -GMA)] exhibited a well-controlled skeletal and well-distributed porous structure. The α-fetoprotein (AFP) immunoaffinity monolithic polymer array prepared by the immobilization of AFP on epoxy-functionalized monolithic arrays was used as an immunosensor for chemiluminescent AFP detection. X-ray photoelectron spectroscopy results indicate that the AFP antibody was successfully immobilized on the monolithic poly(BABEA- co -GMA) array. With a noncompetitive immune-response format, the proposed AFP immunoaffinity array was demonstrated as a low-cost, flexible, homogeneous, and stable array for AFP detection. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132 , 41792.