On-point detection of GM rice in 20 minutes with pullulan as CPA acceleration additive

1% (w/v) concentration of pullulan as a cross-priming isothermal amplification (CPA) reaction additive has a great acceleration effect on nucleic acid amplification. Using this property of pullulan, on-point specific detection of nucleic acid by lateral-flow dipstick (LFD) within 20 minutes was achieved with initial DNA template of 750 copies.