Amperometric determination of NADH based on a poly-Ni(II)–curcumin composite film modified glassy carbon electrode

A poly-Ni(II)–curcumin (curcumin: 1,7-bis[4-hydroxy-3-methoxyphenyl]-1,6-heptadiene-3,5-dione) composite film modified electrode was prepared for amperometric determination of β-nicotinamide adenine dinucleotide (NADH). Scanning electron microscopy and Energy Dispersive X-ray spectroscopy were used to examine the morphology and interface property of the Ni(II)–curcumin film. Under optimum conditions, the amperometric detection of NADH provided a wide linear detection range (0.3–300 μM), high sensitivity (230.02 μA mM−1 cm−2) and low limit of detection (LOD = 0.18 μM, S/N = 3). Moreover, the modified electrode showed good stability and reproducibility, indicating that it can improve the analysis signal of NADH in a very convenient way.