A sensitive electrochemical method based on Fenton-induced DNA oxidation for detection of hydroxyl radical

A sensitive and selective biosensor has been developed to detect the hydroxyl radical (˙OH), which was generated through the Fenton reaction. ˙OH could oxidatively damage DNA and cleave it into pieces. Based on this, taking [Ru(NH3)6]3+ as the electrochemical indicator, ˙OH was determined on a gold electrode with self-assembled DNA. The resulting electrochemical biosensor showed a wide linear range of detection from 125 pM to 625 nM with a detection limit of 80 pM and satisfactory selectivity.