Synthesis and Inhibitory Activity Evaluation of 2,6-Disubstituted Purine Derivatives

A series of novel 2,6-disubstituted purine derivatives were designed and synthesized from 2,6-dichloropurine. The structures of target compounds were determined by 1 H-NMR, 13 C-NMR, and HRMS. The synthesized compounds were evaluated for their inhibitory activities against lung cancer cell lines of A549 and liver cancer cell lines of Bel-7402. 2-(4-Benzyloxy-phenylamino)-6-(cyclohexylamino)purine( 3 ), 2-(4-chloro-phenylamino)-6-( n -butylamino)purine ( 5 ), 2-(4-morpholinoamino)-6-(4-hydroxy-phenylamino)purine ( 9 ), and 2-(4- O -galactosyl-phenylamino)-6-(cyclohexylamino)purine ( 12 ) exhibited moderate inhibitory activity.