Optimization of enzyme assisted extraction of Fructus Mori polysaccharides and its activities on antioxidant and alcohol dehydrogenase

In the present study, enzyme assisted extraction of Fructus Mori polysaccharides ( FMPS ) from F. mori using four kinds of enzymes and three compound enzymes were examined. Research found that glucose oxidase offered a better performance in enhancement of the extraction yields of FMPS , antioxidant and activate alcohol dehydrogenase activities. The glucose oxidase assisted extraction process was further optimized by using response surface method ( RSM ) to obtain maximum yield of crude FMPS . The results showed that optimized extraction conditions were ratio of enzyme amount 0.40%, enzyme treated time 38   min, treated temperature 58   °C and liquid–solid radio 11.0. Under these conditions, the mean experimental value of extraction yield (16.16   ±   0.14%) corresponded well with the predicted values and increased 160% than none enzyme treated ones. Pharmacological verification tests showed that F. mori crude polysaccharides had good antioxidant and activate alcohol dehydrogenase activities in vitro.