摘要
Silica-coated, thiol-enriched and zinc-doped CdS quantum dots (SiO 2 -S-Zn-CdS QDs) were fabricated by a sol–gel process and used as fluorescent probe for imaging and monitoring of intracellular Cd(II) and Zn(II) in endophytes in the “turn-on” mode. There is a linear relationship between fluorescence intensity and the concentration of Zn(II) in the 3.5 – 23.2 μM concentration range and with a detection limit of 2.0 μM. The probe also responds well to Cd(II) in the 0.3 – 26 μM concentration range and the detection limit is 0.1 μM. Due to its negligible cytotoxicity, fair sensitivity and good photostability, the quantum dot probe presented here is a promising tool for fluorescent imaging of intracellular Zn(II) or Cd(II). The SiO 2 -S-Zn-CdS QDs “turn-on” fluorescent probe shows superior selectivity for Zn 2+ and Cd 2+
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