Isolation and partial characterization of a neutral polysaccharide from Mosla chinensis Maxim. cv. Jiangxiangru and its antioxidant and immunomodulatory activities

A neutral polysaccharide MP-1 was isolated and purified from Mosla chinensis Maxim. cv. Jiangxiangru. The average molecular weight was 587.53   kDa and it was composed of glucose, galactose , arabinose , mannose and rhamnose in a molar ratio of 16.02:10.01:4.92:4.62:2.23, and trace of fucose . Partial characterization of MP-1 was elucidated by methylation analysis. MP-1 was composed of 1,4-Glc p (31.76%), followed by 1,2-Rha p (17.11%), 1,2,4-Rha p (6.47%), 1,3,4-Gal p (5.05%), 1,4-Man p (3.61%), 1,2,4-Man p (2.55%), 1,5-Ara f (3.10%) and 1,4,6-Glc p (1.53%). The branching points were located at O-2, O-4 or O-6 with α-Ara f (4.01%), α-Rha p (4.31%), α-Fuc p (2.39%), β-Gal p (11.02%) and β-Glc p (4.04%) as terminal residues. MP-1 exhibited a dose-dependent enhancement in antioxidant assays in this study. Immunomodulatory effects of MP-1 were assessed by splenocyte proliferation test and nitric oxide production of mouse peritoneal macrophages.