Synthesis and DNA Cleavage Activity of Piperazine Containing Guanidinoethyl and Hydroxyethyl Side Arms

According to active groups synergetic catalysis principle, a novel phosphodiester receptor 1-( N -guanidinoethyl)-4-( N -hydroxyethyl)-piperazidine hydrochloride was firstly synthesized and the preliminary studies of its DNA cleavage activity. It is characterized and confirmed by methods as 1 H NMR and 13C NMR. A “couple hardness with softness” piperazidine is designed to connect guanidinium group and hydroxyl group; The reserch was showed that the best cleaving conditions was 7.2 in pH; Cleaving DNA by the compound reaction was oxidation process that were proved through the free radicals quenches experiments; The compound can cleaved the pUC 19 DNA by phospholipid transferance was proved by BDNPP.