One-pot preparation of uricase–poly(thiophene-3-boronic acid)–Ptnano composites for high-performance amperometric biosensing of uric acid

Chemical oxidation of glycoprotein-bound thiophene-3-boronic acid (TBA) monomer by relatively mild oxidant chloroplatinate in neutral aqueous solution containing uricase yielded uricase–PTBA–Pt nano bionanocomposite with high enzymatic specific activity on a platinized (Pt plate ) Au electrode, which was then covered by an outer-layer chitosan (CS) film to fabricate a CS/uricase–PTBA–Pt nano /Pt plate /Au electrode. This electrode exhibited a linear amperometric response to uric acid concentration from 5   μM to 1.2   mM with a sensitivity of 134   μA   mM −1   cm −2 , a limit of detection ( S / N   =   3) of 1   μM, and excellent operation/storage stability, which also worked well in serum samples.