Electrochemical immunoassay for subgroup J of avian leukosis viruses using a glassy carbon electrode modified with a film of poly (3-thiophene boronic acid), gold nanoparticles, graphene and immobilized antibody

We have modified a glassy carbon electrode (GCE) with a film of poly(3-thiophene boronic acid), gold nanoparticles and graphene, and an antibody (Ab) was immobilized on its surface through the covalent bond formed between the boronic acid group and the glycosyl groups of the Ab. Subgroup J of avian leukosis viruses (ALV-J) were electrochemically determined with the help of this electrode. There is a linear relationship between the electron transfer resistance ( R et ) and the concentration of ALV-J in the range from 527 to 3,162 TCID 50 ⋅mL −1 (where TCID 50 is the 50 % tissue culture infective dose). The detection limit is 210 TCID 50 ⋅mL −1 (at an S/N of 3), and the correlation coefficient ( R ) is 0.9964. The electrochemical immunoassay showed good selectivity, stability and reproducibility. Schematic illustration of the stepwise immunosensor fabrication process