Determination of l-phenylalanine on-line based on molecularly imprinted polymeric microspheres and flow injection chemiluminescence

A novel molecular imprinting-chemiluminescence (MIP-CL) sensor for the determination of l -phenylalanine (Phe) using molecularly imprinted polymer (MIP) as recognition element is reported. The Phe-MIP was synthesized using acrylamide (AM) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker, 2,2-azobisisobutyronitrile (AIBN) as initiator and the polymers’ properties were characterized. Then the synthesized MIP was employed as recognition element by packing into flow cell to establish a novel flow injection CL sensor. The CL intensity responded linearly to the concentration of Phe in the range 1.3   ×   10 −6 to 5.44   ×   10 −4   mol/L with a detection limit of 6.23   ×   10 −7   mol/L (3 σ ), which is lower than that of conventional methods. The sensor is reusable and has a great improvement in sensitivity and selectivity for CL analysis. As a result, the new MIP-CL sensor had been successfully applied to the determination of Phe in samples.